Our qPCR Expertise
Knowledge and experience, the core of BioSistemika’s qPCR Experience workshops has its roots in the first and most experienced qPCR centre in Slovenia, the qPCR centre at the National Institute of Biology. We are still involved in qPCR research work there and present in the international qPCR community since 2002 as seen from our selected scientific publications below.
All instructors on qPCR Experience workshops are well experienced in complete qPCR workflow, have a broad overview over technology and its future directions and are trained to work in laboratories that have quality assurance and follow strict rules of ISO standards. Part of our team was actively involved in setting-up such standards in a routine molecular diagnostic laboratory, in pertaining (qPCR) method validation and accreditaion procedures and are trained internal auditors for ISO 9001 and ISO/IEC 17025.
Having practical experience in scientific, diagnostic, industrial research and in presentation skills either for international scientific community or for regional specialised communities and internal educations our team is able to present the qPCR topics, research work and address trouble-shooting questions in a straightforward and professional manner. Since 2006 members of our team have organized hands-on practical qPCR workshops for national and international partners of National Institute of Biology (see list of our past workshops and our customers).
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Selected real-time PCR publications by BioSistemika's team in scientific journals and monographs
ŽEL Jana, MILAVEC Mojca, MORISSET Dany, PLAN Damien, EEDE G. van den, GRUDEN Kristina. How to reliably test for GMOs, (Springer briefs in food, health, and nutrition). New York [etc.]: Springer, 2012. X, 100 pp., ilustr. ISBN 978-1-4614-1389-9.
ČEPIN Urška, GUTIERREZ-AGUIRRE Ion, BALAŽIC Leonida, POMPE NOVAK Maruša, GRUDEN Kristina, RAVNIKAR Maja. A one-step reverse transcription real-time PCR assay for the detection and quantitation of Grapevine fanleaf virus. J. virol. methods., 2010, vol. 170, issues 1-2, p. 47-56. http://dx.doi.org/10.1016/j.jviromet.2010.08.018
HREN Matjaž, DREO Tanja, ERJAVEC Jana, NIKOLIĆ Petra, BOBEN Jana, GRUDEN Kristina, DERMASTIA Marina, CAMLOH Marjana, RAVNIKAR Maja. Real-time PCR detection methods for economically important grapevine related bacteria. In: DELROT, Serge (ed.), MEDRANO, Hipólito (ed.), OR, Etti (ed.), BAVARESCO, Luigi (ed.), GRANDO, Stella (ed.). Methodologies and results in grapevine research. Dordrecht: Springer, 2010, p. 229-246.
NIKOLIĆ Petra, MEHLE Nataša, GRUDEN Kristina, RAVNIKAR Maja, DERMASTIA Marina. A panel of real-time PCR assays for specific detection of three phytoplasmas from the apple proliferation group. Mol. cell. probes, 2010, vol. 24, p. 303-309. http://dx.doi.org/10.1016/j.mcp.2010.06.005, doi: 10.1016/j.mcp.2010.06.005.
PAPAZOVA Nina, ZHANG David, GRUDEN Kristina, VOJVODA Jana, YANG Litao, BUH GAŠPARIČ Meti, BLEJEC Andrej, FOUILLOUX Stephane, DE LOOSE Marc, TAVERNIERS Isabel. Evaluation of the reliability of maize reference assays for GMO quantification. Anal. bioanal. chem., 2010, vol. 396, no. 6, p. 2189-2201. http://dx.doi.org/10.1007/s00216-009-3386-4
MORISSET Dany, DEMŠAR Tina, GRUDEN Kristina, VOJVODA Jana, ŠTEBIH Dejan, ŽEL Jana. Detection of genetically modified organisms-closing the gaps : to the editor. Nat. biotechnol., 2009, vol. 27, no. 8, 9 pp. http://dx.doi.org/10.1038/nbt0809-700, doi: 10.1038/nbt0809-700. [COBISS.SI-ID 2059087]
HREN Matjaž, NIKOLIĆ Petra, ROTTER Ana, BLEJEC Andrej, TERRIER Nancy, RAVNIKAR Maja, DERMASTIA Marina, GRUDEN Kristina. 'Bois noir' phytoplasma induces significant reprogramming of the leaf transcriptome in the field grown grapevine. BMC Genomics, 2009, vol. 10, no. 460. http://www.biomedcentral.com/1471-2164/10/460/abstract, doi: 10.1186/1471-2164-10-460.
BAEBLER Špela, KREČIČ STRES Hana, ROTTER Ana, KOGOVŠEK Polona, CANKAR Katarina, KOK Esther, GRUDEN Kristina, KOVAČ Maja, ŽEL Jana, POMPE NOVAK Maruša, RAVNIKAR Maja. PVYNTN elicits a diverse gene expression response in different potato genotypes in the first 12 h after inoculation. Mol. plant pathol., 2009, vol. 10, no. 2, p. 263-275. http://dx.doi.org/10.1111/J.1364-3703.2008.00530.X
HREN Matjaž, NIKOLIĆ Petra, ROTTER Ana, BLEJEC Andrej, TERRIER Nancy, RAVNIKAR Maja, DERMASTIA Marina, GRUDEN Kristina. 'Bois noir' phytoplasma induces significant reprogramming of the leaf transcriptome in the field grown grapevine. BMC Genomics, 2009, vol. 10, no. 460. http://www.biomedcentral.com/1471-2164/10/460/abstract
VIRANT-KLUN Irma, VOGLER Andrej, ŠINKOVEC Jasna, HREN Matjaž, GRUDEN Kristina, MALIČEV Elvira, MEDEN-VRTOVEC Helena. Ovarijska neplodnost, matične celice in oogeneza in vitro = Ovarian infertility, stem cells and oogenesis in vitro. In: 4. Kongres ginekologov in porodničarjev Slovenije z mednarodno udeležbo, Portorož, 11. do 13. november 2009, (Zdravniški Vestnik, vol. 78, Suppl 1). Ljubljana: Slovensko zdravniško društvo, 2009, p. I-57 - I-61. http://szd.si/user_files/vsebina/Zdravniski_Vestnik/2009/Suplement/057-061.pdf (publication is in Slovene language)
KOGOVŠEK Polona, GOW Lisa, POMPE NOVAK Maruša, GRUDEN Kristina, FOSTER Gary D., BOONHAM Neil, RAVNIKAR Maja. Single-step RT real-time PCR for sensitive detection and discrimination of potato virus Y isolates. J. virol. methods., 2008, vol. 149, no. 1, p. 1-11.
GRUDEN Kristina, POMPE NOVAK Maruša, BAEBLER Špela, KREČIČ STRES Hana, TOPLAK Nataša, HREN Matjaž, KOGOVŠEK Polona, GOW Lisa, FOSTER Gary D., BOONHAM Neil, RAVNIKAR Maja. Expression microarrays in plant-virus interaction. In: FOSTER, Gary D. (ed.). Plant virology protocols: from viral sequence to protein function, (Methods in molecular biology, 451). 2nd ed. Totowa: Humana Press, 2008, p. 583-613.
GUTIERREZ-AGUIRRE Ion, STEYER Andrej, BOBEN Jana, GRUDEN Kristina, POLJŠAK-PRIJATELJ Mateja, RAVNIKAR Maja. Sensitive detection of multiple rotavirus genotypes with a single reverse transcription-real-time quantitative PCR assay. J. Clin. Microbiol., 2008, vol. 46, no. 8, p. 2547-2554.
ŽEL Jana, MAZZARA Marco, SAVINI Cristian, CORDEIL Stephane, CAMLOH Marjana, ŠTEBIH Dejan, CANKAR Katarina, GRUDEN Kristina, MORISSET Dany, EEDE G. van den. Method validation and quality management in the flexible scope of accreditation : an example of laboratories testing for genetically modified organisms. Food analytical methods, 2008, issue 2, vol. 1, p. 61-72. http://dx.doi.org/10.1007/s12161-008-9016-5
BUH GAŠPARIČ Meti, CANKAR Katarina, ŽEL Jana, GRUDEN Kristina. Comparision of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms. BMC Biotechnol, 2008, vol. 8, no. 26, p. 1-12. http://dx.doi.org/10.1186/1472-6750-8-26
HREN Matjaž, BOBEN Jana, ROTTER Ana, KRALJ NOVAK Petra, GRUDEN Kristina, RAVNIKAR Maja. Real-time PCR detection systems for Flavescence dorée and Bois noir phytoplasmas in grapevine : comparision with conventional PCR detection and application in diagnostics. Plant Pathol., 2007, vol. 56, p. 785-796.
CANKAR Katarina, ŠTEBIH Dejan, DREO Tanja, ŽEL Jana, GRUDEN Kristina. Critical points of DNA quantification by real-time PCR-effects of DNA extraction method and sample matrix on quantification of genetically modified organisms. BMC Biotechnol, 2006, vol. 6, no. 37.
CANKAR Katarina, RAVNIKAR Maja, ŽEL Jana, GRUDEN Kristina, TOPLAK Nataša. Real-time polymerase chain reaction detection of Cauliflower mosaic virus to complement the 35S screening assay for genetically modified organisms. J. AOAC Int., 2005, vol. 88, no. 3, p. 814-822.
All instructors on qPCR Experience workshops are well experienced in complete qPCR workflow, have a broad overview over technology and its future directions and are trained to work in laboratories that have quality assurance and follow strict rules of ISO standards. Part of our team was actively involved in setting-up such standards in a routine molecular diagnostic laboratory, in pertaining (qPCR) method validation and accreditaion procedures and are trained internal auditors for ISO 9001 and ISO/IEC 17025.
Having practical experience in scientific, diagnostic, industrial research and in presentation skills either for international scientific community or for regional specialised communities and internal educations our team is able to present the qPCR topics, research work and address trouble-shooting questions in a straightforward and professional manner. Since 2006 members of our team have organized hands-on practical qPCR workshops for national and international partners of National Institute of Biology (see list of our past workshops and our customers).
Image below shows a complete workflow related to gene expression analysis by real-time PCR, one of the most demanding applications of real-time PCR. It contains several stages each requesting a specific set of skills. In qPCR Experience workshops all critical elements of the workflow are presented and discussed by instructors experienced in qPCR.
Selected real-time PCR publications by BioSistemika's team in scientific journals and monographs
ŽEL Jana, MILAVEC Mojca, MORISSET Dany, PLAN Damien, EEDE G. van den, GRUDEN Kristina. How to reliably test for GMOs, (Springer briefs in food, health, and nutrition). New York [etc.]: Springer, 2012. X, 100 pp., ilustr. ISBN 978-1-4614-1389-9.
ČEPIN Urška, GUTIERREZ-AGUIRRE Ion, BALAŽIC Leonida, POMPE NOVAK Maruša, GRUDEN Kristina, RAVNIKAR Maja. A one-step reverse transcription real-time PCR assay for the detection and quantitation of Grapevine fanleaf virus. J. virol. methods., 2010, vol. 170, issues 1-2, p. 47-56. http://dx.doi.org/10.1016/j.jviromet.2010.08.018
HREN Matjaž, DREO Tanja, ERJAVEC Jana, NIKOLIĆ Petra, BOBEN Jana, GRUDEN Kristina, DERMASTIA Marina, CAMLOH Marjana, RAVNIKAR Maja. Real-time PCR detection methods for economically important grapevine related bacteria. In: DELROT, Serge (ed.), MEDRANO, Hipólito (ed.), OR, Etti (ed.), BAVARESCO, Luigi (ed.), GRANDO, Stella (ed.). Methodologies and results in grapevine research. Dordrecht: Springer, 2010, p. 229-246.
NIKOLIĆ Petra, MEHLE Nataša, GRUDEN Kristina, RAVNIKAR Maja, DERMASTIA Marina. A panel of real-time PCR assays for specific detection of three phytoplasmas from the apple proliferation group. Mol. cell. probes, 2010, vol. 24, p. 303-309. http://dx.doi.org/10.1016/j.mcp.2010.06.005, doi: 10.1016/j.mcp.2010.06.005.
PAPAZOVA Nina, ZHANG David, GRUDEN Kristina, VOJVODA Jana, YANG Litao, BUH GAŠPARIČ Meti, BLEJEC Andrej, FOUILLOUX Stephane, DE LOOSE Marc, TAVERNIERS Isabel. Evaluation of the reliability of maize reference assays for GMO quantification. Anal. bioanal. chem., 2010, vol. 396, no. 6, p. 2189-2201. http://dx.doi.org/10.1007/s00216-009-3386-4
MORISSET Dany, DEMŠAR Tina, GRUDEN Kristina, VOJVODA Jana, ŠTEBIH Dejan, ŽEL Jana. Detection of genetically modified organisms-closing the gaps : to the editor. Nat. biotechnol., 2009, vol. 27, no. 8, 9 pp. http://dx.doi.org/10.1038/nbt0809-700, doi: 10.1038/nbt0809-700. [COBISS.SI-ID 2059087]
HREN Matjaž, NIKOLIĆ Petra, ROTTER Ana, BLEJEC Andrej, TERRIER Nancy, RAVNIKAR Maja, DERMASTIA Marina, GRUDEN Kristina. 'Bois noir' phytoplasma induces significant reprogramming of the leaf transcriptome in the field grown grapevine. BMC Genomics, 2009, vol. 10, no. 460. http://www.biomedcentral.com/1471-2164/10/460/abstract, doi: 10.1186/1471-2164-10-460.
BAEBLER Špela, KREČIČ STRES Hana, ROTTER Ana, KOGOVŠEK Polona, CANKAR Katarina, KOK Esther, GRUDEN Kristina, KOVAČ Maja, ŽEL Jana, POMPE NOVAK Maruša, RAVNIKAR Maja. PVYNTN elicits a diverse gene expression response in different potato genotypes in the first 12 h after inoculation. Mol. plant pathol., 2009, vol. 10, no. 2, p. 263-275. http://dx.doi.org/10.1111/J.1364-3703.2008.00530.X
HREN Matjaž, NIKOLIĆ Petra, ROTTER Ana, BLEJEC Andrej, TERRIER Nancy, RAVNIKAR Maja, DERMASTIA Marina, GRUDEN Kristina. 'Bois noir' phytoplasma induces significant reprogramming of the leaf transcriptome in the field grown grapevine. BMC Genomics, 2009, vol. 10, no. 460. http://www.biomedcentral.com/1471-2164/10/460/abstract
VIRANT-KLUN Irma, VOGLER Andrej, ŠINKOVEC Jasna, HREN Matjaž, GRUDEN Kristina, MALIČEV Elvira, MEDEN-VRTOVEC Helena. Ovarijska neplodnost, matične celice in oogeneza in vitro = Ovarian infertility, stem cells and oogenesis in vitro. In: 4. Kongres ginekologov in porodničarjev Slovenije z mednarodno udeležbo, Portorož, 11. do 13. november 2009, (Zdravniški Vestnik, vol. 78, Suppl 1). Ljubljana: Slovensko zdravniško društvo, 2009, p. I-57 - I-61. http://szd.si/user_files/vsebina/Zdravniski_Vestnik/2009/Suplement/057-061.pdf (publication is in Slovene language)
KOGOVŠEK Polona, GOW Lisa, POMPE NOVAK Maruša, GRUDEN Kristina, FOSTER Gary D., BOONHAM Neil, RAVNIKAR Maja. Single-step RT real-time PCR for sensitive detection and discrimination of potato virus Y isolates. J. virol. methods., 2008, vol. 149, no. 1, p. 1-11.
GRUDEN Kristina, POMPE NOVAK Maruša, BAEBLER Špela, KREČIČ STRES Hana, TOPLAK Nataša, HREN Matjaž, KOGOVŠEK Polona, GOW Lisa, FOSTER Gary D., BOONHAM Neil, RAVNIKAR Maja. Expression microarrays in plant-virus interaction. In: FOSTER, Gary D. (ed.). Plant virology protocols: from viral sequence to protein function, (Methods in molecular biology, 451). 2nd ed. Totowa: Humana Press, 2008, p. 583-613.
GUTIERREZ-AGUIRRE Ion, STEYER Andrej, BOBEN Jana, GRUDEN Kristina, POLJŠAK-PRIJATELJ Mateja, RAVNIKAR Maja. Sensitive detection of multiple rotavirus genotypes with a single reverse transcription-real-time quantitative PCR assay. J. Clin. Microbiol., 2008, vol. 46, no. 8, p. 2547-2554.
ŽEL Jana, MAZZARA Marco, SAVINI Cristian, CORDEIL Stephane, CAMLOH Marjana, ŠTEBIH Dejan, CANKAR Katarina, GRUDEN Kristina, MORISSET Dany, EEDE G. van den. Method validation and quality management in the flexible scope of accreditation : an example of laboratories testing for genetically modified organisms. Food analytical methods, 2008, issue 2, vol. 1, p. 61-72. http://dx.doi.org/10.1007/s12161-008-9016-5
BUH GAŠPARIČ Meti, CANKAR Katarina, ŽEL Jana, GRUDEN Kristina. Comparision of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms. BMC Biotechnol, 2008, vol. 8, no. 26, p. 1-12. http://dx.doi.org/10.1186/1472-6750-8-26
HREN Matjaž, BOBEN Jana, ROTTER Ana, KRALJ NOVAK Petra, GRUDEN Kristina, RAVNIKAR Maja. Real-time PCR detection systems for Flavescence dorée and Bois noir phytoplasmas in grapevine : comparision with conventional PCR detection and application in diagnostics. Plant Pathol., 2007, vol. 56, p. 785-796.
CANKAR Katarina, ŠTEBIH Dejan, DREO Tanja, ŽEL Jana, GRUDEN Kristina. Critical points of DNA quantification by real-time PCR-effects of DNA extraction method and sample matrix on quantification of genetically modified organisms. BMC Biotechnol, 2006, vol. 6, no. 37.
CANKAR Katarina, RAVNIKAR Maja, ŽEL Jana, GRUDEN Kristina, TOPLAK Nataša. Real-time polymerase chain reaction detection of Cauliflower mosaic virus to complement the 35S screening assay for genetically modified organisms. J. AOAC Int., 2005, vol. 88, no. 3, p. 814-822.