qPCR Experience workshop: Real-time PCR applications in medical research and diagnostics, Ljubljana, 23.-25.3. 2011 (FULLY BOOKED)
Real-time PCR technology is becoming a must technique in every lab since it allows for sensitive, accurate and high-throughput method applicable in various areas of life sciences, from pathogen or GMO determination and quantification, gene expression studies, genotyping etc. If you haven't mastered it yet, here's your chance. The hands-on 3-day workshop will cover qPCR theory, practical guidance on sample preparation including blood samples and single cell analysis as well as hands-on lab and data analysis sessions.
The qPCR Experience workshop will feature three guest specialists/lecturers, prof. dr. Kristina Gruden, dr. Srdjan Novaković and dr. Vida Stegel experienced researchers in the fields of gene expression studies, quality control in diagnostic laboratories and medical diagnostics. Their expert and practical experience will contribute to the applied orientation of the workshop.
The workshop is organised in colaboration with National Institute of Biology.
The hands-on lab sessions will take place at the Institute's qPCR centre, the first and the best equipped qPCR centre in the country and region.
Workshop level: advanced (basic theoretical and practical PCR knowledge required)
Workshop programme:
Wednesday, March 23 (9 am - 5 pm):
• Real-time PCR principles and applications
• Chemistries and equipment
• Sample preparation
• Primer and probe design
• Experimental setup practical
Thursday, March 24 (9 am - 4 pm)
• Quality assurance compliant laboratory workflow
• Lab work: sample preparation and reaction setup
• Introduction to data analysis
• Data Analysis: SDS, melting curve analysis
• MIQE guidelines (minimum information for publication of quantitative real-time PCR experiments)
• HRM and SNP analysis in medical diagnostics (by V. Stegel)
Friday, March 25 (9 am - 1 pm)
• Single-cell real time PCR
• Relative quantification
• Data analysis practical
• Validations and quality control (by K. Gruden)
• Wrap-up and discussion
Instructors: dr. Špela Baebler, Urška Čepin, dr. Matjaž Hren
Guest lecturers: prof. dr. Kristina Gruden (NIB), dr. Vida Stegel (IO)
Venue:
National Institute of Biology
Večna pot 111
SI-1000 Ljubljana
Slovenia
Fee: 960€ (VAT incl.)
• Discounts: academia 20%, students (50%), groups (inquire)
• Course materials, coffee break refreshments and lunches included
Registration:
Registration is closed - the workshop is fully booked. Please take a look at our future workshops.
In order to ensure high quality of the workshop there are only 12 places available; registration is on a first-come first-served basis. Please fill and send the e-registration form (below) until March 16, 2011 and we will contact you with the payment details. If you require any additional information or help regarding accomodation in Ljubljana, do not hesitate to contact us at info@biosistemika.com.
Guest lecturers:
prof. dr. Kristina Gruden
head of Omics unit at Department of Biotechnology and Systems Biology at National Institute of Biology
Prof. dr. Kristina Gruden is one of the leading molecular biology researchers in Slovenia. One of the fields of her research and educational work is development and validation of molecular diagnostic methods for quantification of genetically modified organisms (GMO) based on real-time PCR. Her contribution to the outstanding quality and high level of molecular diagnostic expertise (including real-time PCR) at the Department is enormous. And exactly the high quality and level of molecular diagnostic expertise (including real-time PCR) at the Department lead to the authorisation of the NIB as the National reference laboratory for GMO detection in in feed and food by the Ministry of healthy and by Ministry of agriculture, forestry and food.
In this »Real-time PCR gene applications in medical research and diagnostics« workshop prof. dr. Kristina Gruden will focus on the introduction of new real-time PCR based methods of detection into the molecular laboratory, including the validation of such methods and give general tips on quality management in the diagnostic laboratories that use real-time PCR based diagnostic methods. Her practical experience in the first laboratory in Slovenia that was accredited for quantitative real-time PCR based detection methods according to standard ISO17025 (competence of testing and calibration laboratories) will without doubt contribute to high quality of the workshop.
dr. Vida Stegel
researcher at Department of molecular diagnostics at Institute of Oncology Ljubljana, national comprehensive cancer center in Slovenia.
Dr. Vida Stegel is actively involved in research and development of molecular based detection methods for identification of hereditary neoplasms (cancers) and other hereditary genetic changes for routine medical diagnostics. She has also vast experiences in routine medical diagnostic analyses of polymorphisms with real-time PCR.
The qPCR Experience workshop will feature three guest specialists/lecturers, prof. dr. Kristina Gruden, dr. Srdjan Novaković and dr. Vida Stegel experienced researchers in the fields of gene expression studies, quality control in diagnostic laboratories and medical diagnostics. Their expert and practical experience will contribute to the applied orientation of the workshop.
The workshop is organised in colaboration with National Institute of Biology.
The hands-on lab sessions will take place at the Institute's qPCR centre, the first and the best equipped qPCR centre in the country and region.Workshop level: advanced (basic theoretical and practical PCR knowledge required)
Workshop programme:
Wednesday, March 23 (9 am - 5 pm):
• Real-time PCR principles and applications
• Chemistries and equipment
• Sample preparation
• Primer and probe design
• Experimental setup practical
Thursday, March 24 (9 am - 4 pm)
• Quality assurance compliant laboratory workflow
• Lab work: sample preparation and reaction setup
• Introduction to data analysis
• Data Analysis: SDS, melting curve analysis
• MIQE guidelines (minimum information for publication of quantitative real-time PCR experiments)
• HRM and SNP analysis in medical diagnostics (by V. Stegel)
Friday, March 25 (9 am - 1 pm)
• Single-cell real time PCR
• Relative quantification
• Data analysis practical
• Validations and quality control (by K. Gruden)
• Wrap-up and discussion
Instructors: dr. Špela Baebler, Urška Čepin, dr. Matjaž Hren
Guest lecturers: prof. dr. Kristina Gruden (NIB), dr. Vida Stegel (IO)
Venue:
National Institute of Biology
Večna pot 111
SI-1000 Ljubljana
Slovenia
Fee: 960€ (VAT incl.)
• Discounts: academia 20%, students (50%), groups (inquire)
• Course materials, coffee break refreshments and lunches included
Registration:
Registration is closed - the workshop is fully booked. Please take a look at our future workshops.
In order to ensure high quality of the workshop there are only 12 places available; registration is on a first-come first-served basis. Please fill and send the e-registration form (below) until March 16, 2011 and we will contact you with the payment details. If you require any additional information or help regarding accomodation in Ljubljana, do not hesitate to contact us at info@biosistemika.com.
Guest lecturers:
prof. dr. Kristina Gruden
head of Omics unit at Department of Biotechnology and Systems Biology at National Institute of Biology
Prof. dr. Kristina Gruden is one of the leading molecular biology researchers in Slovenia. One of the fields of her research and educational work is development and validation of molecular diagnostic methods for quantification of genetically modified organisms (GMO) based on real-time PCR. Her contribution to the outstanding quality and high level of molecular diagnostic expertise (including real-time PCR) at the Department is enormous. And exactly the high quality and level of molecular diagnostic expertise (including real-time PCR) at the Department lead to the authorisation of the NIB as the National reference laboratory for GMO detection in in feed and food by the Ministry of healthy and by Ministry of agriculture, forestry and food.
In this »Real-time PCR gene applications in medical research and diagnostics« workshop prof. dr. Kristina Gruden will focus on the introduction of new real-time PCR based methods of detection into the molecular laboratory, including the validation of such methods and give general tips on quality management in the diagnostic laboratories that use real-time PCR based diagnostic methods. Her practical experience in the first laboratory in Slovenia that was accredited for quantitative real-time PCR based detection methods according to standard ISO17025 (competence of testing and calibration laboratories) will without doubt contribute to high quality of the workshop.
dr. Vida Stegel
researcher at Department of molecular diagnostics at Institute of Oncology Ljubljana, national comprehensive cancer center in Slovenia.
Dr. Vida Stegel is actively involved in research and development of molecular based detection methods for identification of hereditary neoplasms (cancers) and other hereditary genetic changes for routine medical diagnostics. She has also vast experiences in routine medical diagnostic analyses of polymorphisms with real-time PCR.