Coming qPCR Experience® workshops


Would you like to have a specific focus of real-time PCR workshops?

You are welcome to contribute ideas and suggestions. Please send them to info@biosistemika.com.

Download a pdf flyer of Coming qPCR Experience Workshops here

Focus

 

Theoretical overview

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General qPCR workshop with focus on medical diagnostics of pathogens and SNPs. Workshop is in the process of registration for the credits of Slovenian chamber of laboratory medicine.
* qPCR technology overview
* chemistries & equipment overview
* qPCR applications
* laboratory workflow
* sample preparation
* design of qPCR methods
* validation of qPCR methods
* qPCR reaction setup
* quantification strategies for
   gene expression analyses
 

Hands on laboratory
work

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Hands on data
analysis on
computers

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* demonstration of laboratory workflow
   & lab organisation
* experiment setup
* preparation of samples including
   single cell protocols
* reaction mixture preparation
   and pipetting
* loading samples onto plate
* qPCR instrument setup & run

* preliminary raw data analysis & QC
* export of data
* analysis & interpretation
   of diagnostic data
* analysis & interpretation
   of quantification data


Spotlight lectures & Invited speakers:

* Invited specialist of lab medicine working on SNP technology.

Target audience: from beginners to advanced qPCR users, suitable also for the QA and lab managers
Duration: 3 days
Language: Slovene

Workshop is OPEN for registration

Further details:
  Real-time PCR technology is becoming a must technique in every lab since it allows for sensitive, accurate and high-throughput method applicable in various areas of life sciences, from pathogen or GMO determination and quantification, gene expression studies, genotyping etc. If you haven't mastered it yes, here's your chance.

The hands-on 3-day workshop will cover qPCR theory, practical guidance on sample preparation including blood samples and single cell analysis as well as hands-on lab and data analysis sessions. Applications in medical diagnostics will be presented by guest lecturer, prof. Dr Borut Peterlin, Dr. med.


  • qPCR data analysis strategies for different applications, Maribor, Slovenia, 26. September 2012

Focus

 

Theoretical overview

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Workshop is a satellite workshop of Genetics 2012 Congress. It will focus on data analysis strategies for different applications of qPCR (diagnostics, gene expression studies, SNPs detection, validation for NGS).
* qPCR technology overview
* qPCR applications (diagnostics,
   gene expression studies, SNPs detection,
   validation for NGS)
* sample preparation & QC
* qPCR reaction setup
* relative qPCR quantification strategies

 

Hands on laboratory
work

LAB-transparent.png  

Hands on data
analysis on
computers

* this workshop contains no hands on laboratory work
* preliminary raw data analyses & QC
* export of data
* relative quantification on qPCR
* interpretation of results
* data analysis of diagnostic results
* data analysis of SNP detection


This is the stallite workshop of the Congress Genetika 2012 (27.9. - 30.9.2012)
Target audience:
from medium to advanced qPCR users
Duration: 1 day
Language: English
Further details: available in February 2012


  • qPCR Experience Boot Camp, Piran, Slovenia, 11.-14. September 2012

Focus

 

Theoretical overview

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Hands-on real-time PCR workshop covering complete laboratory and data analysis workflow with extensive practical sections.

* qPCR technology overview
* chemistries & equipment overview
* qPCR applications
* laboratory workflow
* sample preparation
* design of qPCR methods
* qPCR reaction setup
* relative and absolute quantification

 

Hands on laboratory
work

LAB-transparent.png  

Hands on data
analysis on
computers

* Experiment setup
* preparation & QC of samples
* reaction mixture preparation and pipetting
* loading samples onto plate
* qPCR instrument setup & run
* preliminary raw data analyses &QC
* export of data
* relative quantification strategies
* interpretation of results
* analysis & interpretation
   of diagnostic data


Spotlight lectures & Invited speakers: Prof. Dr. Michael Pfaffl, Technische Universität München, Munich, Germany; a world renowned real-time PCR expert, author of one of the relative quantification methods, co-organiser of annual International qPCR Symposiums in Freising-Weihenstephan (Germany) and curator of the www.gene-quantification.info portal and CEO of bioMCC (formerly bioEPS).

Target audience: from beginners to advanced qPCR users
Duration: 4 days
Language: English
Further details: available in February 2012


  • Plant Pathology diagnostics, Ljubljana, Slovenia, November/December 2012

Focus

 

Theoretical overview

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Hands-on workshop will cover real-time PCR in diagnostics in the field of plant pathology.

* qPCR technology overview
* chemistries & equipment overview
* qPCR applications
* laboratory workflow
* sample preparation
* design of qPCR methods
* validation of qPCR methods
   underEPPO standards
* qPCR reaction setup
* quantification strategies for
   gene expression analyses

 

Hands on laboratory
work

LAB-transparent.png  

Hands on data
analysis on
computers

* demonstration of laboratory workflow
   & lab organisation
* demonstration of homogenisation
   & nucleic acid isolation strategies
* experiment setup
* preparation & QC of samples
* reaction mixture preparation
   and pipetting
* loading samples onto plate
* qPCR instrument setup & run

* preliminary raw data analysis & QC
* export of data
* analysis & interpretation
   of diagnostic data
* analysis & interpretation
   of quantification data



Spotlight lectures & Invited speakers: as in Plant Pathology diagnostic workshop 2011 we will invite guest lecturers with attractive topics and discussions

Target audience: plant pathologists working with microbiology and molecular biology techniques, the QA specialists and lab managers
Duration: 4 days
Language: English
Further details: available in March 2012


Would you like to have a specific focus of the workshops?
You are welcome to contribute ideas and suggestions. Please send them to info@biosistemika.com.

We reserve the right to change the program and details of workshops.